L1CAM Knockout cell line (MDCK)

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L1CAM Knockout cell line (MDCK) Order

L1CAM Knockout cell line (MDCK)
Catalog#
YKO-R073
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
L1CAM Knockout cell line (MDCK)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
L1CAM Knockout cell line (MDCK)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:4
Species
Dog
Gene Name
L1CAM
Gene ID
492244
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%MEM+10%FBS
Accession
CVCL_E0YY(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 2 (based on transcript L1CAM-201)
Validation: PCR+Sanger Sequencing
L1CAM Knockout cell line (MDCK) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:MDCK		 STR Info (Cell bank)
Cell Line:NALM-6
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 913913
D7S820 811811
D16S539 10111011
VWA 15161516
TH01 8989
AMEL XYXY
TPOX 810810
CSF1PO 13131313
D12S391 2021
FGA 2223
D2S1338 1724
D21S11 2729
D18S51 1215
D8S1179 1213
D3S1358 1617
D6S1043 1113
PENTAE 1111
D19S433 1315
PENTAD 814
D1S1656 111217.0
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the L1CAM gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the MDCK cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, L1CAM gene knockout L1CAM Knockout cell line (MDCK) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
L1CAM Knockout cell line (MDCK)
L1CAM Knockout cell line (MDCK)
Catalog#
YKO-R073
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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